Sami Khedira and Claudio Marchisio have been ruled out of Tuesday's Champions League game against Barcelona, Juventus head coach Massimiliano Allegri has confirmed.
Neither midfielder played any part in Saturday's 3-0 Serie A win against Chievo and they will not recover in time for Juve's opening Group D fixture.
Juve could also be without Giorgio Chiellini, with the defender struggling to recover from a calf injury in time for the trip to Camp Nou.
"Khedira and Marchisio are definitely out," Allegri told reporters. "Khedira returned from international duty, where he had a knock to the knee, rested for a few days and played 30 minutes.
"The day after, he felt pain again. Basically, it will take a week or so to have him back."
Mario Mandzukic came off with a knock against Chievo, but Allegri is hoping the Croatian and full-back Alex Sandro will be available.
"He has taken a calf on his calf, there should be nothing serious," Allegri said of Mandzukic. "Sandro had a fever, I hope he is already better."
Grande vittoria di squadra, avanti cosí!
Gran victoria de equipo! Seguimos así!
Great team, great win! Let's go ahead pic.twitter.com/stjyFZ3pSG
— Paulo Dybala (@PauDybala_JR) September 9, 2017
Juve knocked Barca out of the Champions League at the quarter-final stage last season, winning 3-0 on aggregate, although they were later outclassed by Real Madrid in the Cardiff final.
And with Chiellini a doubt and Leonardo Bonucci having been sold to Serie A rivals AC Milan in a shock move, Andrea Barzagli could be the only remaining member of the 'BBC' defence that kept two clean sheets against Barcelona's feted 'MSN' attack.
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"It's game of great prestige," said Barzagli. "Compared to five months ago, the stakes are different.
"Then it was a quarter-final and now it is a game for three important group stage points. It will be a tasty game, to show everyone that this year we want to move on."