The actress, 48, is now at home and "doing well," a rep for the couple confirms to PEOPLE.
Wife Ellen DeGeneres took de Rossi to the hospital on Friday.
For more on Portia de Rossi's hospitalization, listen below to the episode of PEOPLE Every Day.
"Ellen rushed Portia to the hospital on Friday night, she was in a lot of pain," a source tells PEOPLE. "It was appendicitis and she had surgery."
Appendicitis is a common condition in which the lining of the appendix is blocked and becomes inflamed, according to the Mayo Clinic. It causes intense pain, and if not treated quickly — typically with surgery to remove the appendix — the organ can burst.
The source added that de Rossi is "now resting at home and Ellen is taking care of her. It was a scare at first, but all good now."
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The couple, who wed in August 2008 after same-sex marriage was legalized in California, have helped each other through ups and downs in their health and their work lives. de Rossi was by DeGeneres' side throughout the pandemic, during which the talk show host tested positive for COVID-19.
Ari and Louise Ellen DeGeneres and Portia de Rossi
"We've grown together as a couple, and we really consider each other and put our relationship first," de Rossi told PEOPLE in February of their marriage. "By doing that, you become a lot more solid. I can't imagine spending time with anyone but her."
Added DeGeneres: "We think the same things, say the same things at the same time, finish each other's sandwiches — no, sentences. We're in a rhythm that's easy."